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NIKE Hypervenom Phelon Indoor (Clearwater/Blue Lagoon) 0OQdmY9R82 NIKE Hypervenom Phelon Indoor (Clearwater/Blue Lagoon) 0OQdmY9R82 NIKE Hypervenom Phelon Indoor (Clearwater/Blue Lagoon) 0OQdmY9R82 NIKE Hypervenom Phelon Indoor (Clearwater/Blue Lagoon) 0OQdmY9R82 NIKE Hypervenom Phelon Indoor (Clearwater/Blue Lagoon) 0OQdmY9R82
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Mar 30, 2018

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Jul 7, 2018 - 5:31am

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Jul 4, 2018 - 10:02pm

Here's to life, liberty and the pursuit of happiness! Happy Fourth of July!


Jul 4, 2018 - 10:00am

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Jul 4, 2018 - 6:41am

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Jul 4, 2018 - 5:35am

Don’t be a part of that statistic and be extra careful this year!


Jul 3, 2018 - 6:30am

It seems obvious, but you would be surprised…


Jul 2, 2018 - 5:25am

Watch out for the silent culprit – the sparkler. They get extremely hot (up to 1,800°F).


Jul 1, 2018 - 5:20am

Kids should never play with fireworks – they send more than 10,000 people to the ER every year.


Jun 30, 2018 - 5:31am

To cope with stress and depression, one Marine has turned to yoga as a source of comfort and calm. He now teaches y…


Jun 30, 2018 - 1:23am

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Jun 30, 2018 - 12:02am

Go Fund Me Page for those not attending

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Sippin’, Smokin’ Singin’ Saturday Night
Saturday, July 23, 2016 - 6:00pm to 9:00pm
Jowler Creek Winery
Tipple Hill Winery Vineyard- Music
Saturday, July 23, 2016 - 7:00pm to 9:30pm
Tipple Hill Winery Vineyard

Phil Vandelwill be playing young country and your favorite hits.

International Wine Tasting at Riverwood Winery
Sunday, July 24, 2016 - 12:00pm to 4:00pm
Riverwood Winery

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Tipple Hill Winery Vineyard
Destination.Z Live at Blumenhof Winery
Sunday, July 24, 2016 - 2:00pm to 5:00pm
Blumenhof Winery

Destination.Z (Rock Band) will perform at Blumenhof Winery in Dutzow, MOon Sunday, July 24 from 2-5pm. Event URL: Muk Luks Womens Delanie Open Purple Slipper Open Purple MVUsWON

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Thursday, July 28, 2016 - 7:00pm to 9:00pm
Jowler Creek Winery

Looking for a good excuse to get together with your girlfriends to whine…and drink some wine? At this month’s “Women Who Wine” event you’ll be able to visit with friends, make a twine-wrapped chalkboard wine bottle to take home, and sip on some wine. Light appetizers will also be served. Cost: $19/person (or $16.15/person for Creek Club members) which includes one alcoholic or non-alcoholic beverage of your choice, light appetizers, and all the supplies you need to make your craft. Reservations and pre-payment are required for this event. Reserve your spot by calling (816) 858-5528.

Stuart Johnson Live at Blumenhof Winery
Friday, July 29, 2016 - 5:00pm to 8:00pm
Blumenhof Winery

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Fig. 4

CD103 DCs specifically induce Vγ6 γδT17 cell proliferation. a After ex vivo staining of cLN cells and gating from total live cells, CD11c cells were gated for calculating total DCs () then from this population CD103 DCs were gated (). Representative of two to three experiments. *** < 0.001. b Gated on total CD103 DCs from WT and IL-17r cLNs, staining of surface activation markers CD80, CD86, and MHC Class II was performed. Both percentages and MFI are shown. Representative of two to three experiments. * < 0.05, *** < 0.001. c 16S rRNA FISH hybridization on frozen tissue sections from WT and IL-17r cLNs. Probe Eub338-cy3 was used and TRIC signal intensity at random, yet the same locations for WT and IL-17r cLN was used to quantify the amount of bacterial RNA in LNs. Representative of three different cLNs from three mice. *** < 0.001. d CFSE-labeled whole cLN cells were depleted of CD103 DCs and cultured for 5days. In conditions of whole cLN cells depleted with CD103 DCs, sorted CD103 DCs were added back in the presence or absence of anti-IL-1β neutralizing mAb (2μg/ml). γδT cell proliferation was shown. Cells were gated on 7AADCD3γδTCR cells. Representative of five experiments. * < 0.05, ** < 0.01, *** < 0.001. . not significant. e Using MoFlo sorter, WT γδ T cells from the LNs and spleens were purified and CFSE labeled then co-cultured with three different IL-17r DC populations (CD103 cLNs, CD103 mLNs, and CD103 cLNs) for 5days. Representative histograms of γδT cell proliferation and summarized percentages of proliferated cells are shown (). Representative of proliferated γδT cells with Vγ4/Vγ1 staining are shown (). Cells were gated on 7AADCD3γδTCR cells. Representative of three experiments. * < 0.05. f 96-well transwell plate was utilized to separate CD103 DCs from cLNs of IL-17r mice from WT γδ T cells to determine if cell-to-cell contact is required. Representative histograms of γδT cell proliferation and summarized percentages of proliferated cells are shown (). Representative of proliferated γδT cells with Vγ4/Vγ1 staining and summarized data are shown (). Cells were gated on 7AADCD3γδTCR cells. Representative of three experiments. *** < 0.001. g After immunostaining of cLNs, gated from total CD11c DCs then gated on CD103 DC population to analyze total CD103 DC % and absolute number. After PMA/ionomycin stimulation and intracellular staining, gated from total γδ T cells then gated on Vγ6 and IL-17 to calculate total Vγ6 γδT17 %. Representative of two experiments. * < 0.05, ** < 0.01, *** < 0.001

To investigate whether CD103 + CD11c + DCs drive γδT17 cell proliferation, we depleted this subset in vitro from the whole cLN using MoFlo sorter and cultured the cells without stimulation for 5days. γδT17 cell proliferation was reduced by 75% after CD103 + CD11c + DCs depletion and restored by adding back CD103 + CD11c + DCs to culture (Fig. 4d ). In addition, IL-1β Ab blockade did not reduce γδT17 proliferation. To further determine whether γδT17 expansion can be induced by cLN CD103 + DCs, WT γδ T cells were co-cultured with different DC populations including CD103 + DCs either from cLNs or mLNs of IL-17r −/− mice (Fig. 4e ). Only CD103 + DCs from the cLNs were able to induce proliferation of the WT γδ T, and this CFSE low population being negative for Vγ4 and Vγ1; thus, Vγ6 + is consistent with ex vivo cLN staining. Consistent with the IL-1β blockade results, γδT cell from IL-1r −/− proliferate well when co-cultured with cLN CD103 + DCs (Additional file Maybest Womens Suede Flat Platform Sneaker Shoes Winter Lace Up Snow Boots Rose YGvMNb
: Figure S2D), suggesting that γδT17 proliferation is not dependent on IL-1r signaling. In addition, neutralizing IL-23 did not significantly impact γδT17 proliferation (Additional file 1 : Figure S2E). To examine whether CD103 + DCs drive γδT17 proliferation in vitro either through cell-to-cell contact or soluble factors, WT γδ T cells were co-cultured with CD103 + DCs from IL-17r −/− mice in a transwell system. The co-cultured γδ T cells with the CD103 + DCs showed strong proliferation, predominately Vγ6. However, when separated from the CD103 + DCs, the WT γδ T cells did not proliferate (Fig. Jerusalem Sandals Mens The Good Shepard Buckled ToeRing Sandal Tan T8vwis1
), suggesting that a cell-to-cell interaction between DCs, specifically CD103 + DCs and γδT17 cells is required for the induction of their proliferation. To determine whether CD103 + DCs drive Vγ6 γδT17 expansion in vivo, IL-17r −/− mice were backcrossed with IRF8 −/− mice, significant reduction in CD103 + DCs [ InterestPrint Womens Snow Boots Unique Designed Comfort Winter Boots Multi 11 O97vFx
]. IL-17r −/− IRF8 −/− mice showed a reduction in total CD103 + DC % and absolute number which correlated with a large reduction in Vγ6 γδT17 in the cLNs, bLNs, and spleen but not the mLNs (Fig. 4g ). This suggests that in vivo CD103 + CD11c + DCs are important in the monoclonal expansion of Vγ6 γδT17 in IL-17r −/− mice.


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